Table 1

Protein profile alterations in breast cancer and under radiotherapy
Spot number Access number Accession name Protein name Functional group number Classification, references relevant for functional groups 19, 20 and 21 Profile alterations versus controls Alterations under radio-therapy
CATEGORY A: significantly (T≤0.1) altered expression profiles in patients versus controls
112-116 P04040 CATA_HUMAN Catalase 5, 9, 10, 11, 14, 18, 19, 20, 21 anti-oxidant defence and detoxification protein [37-42] homogeneous suppression 3x T=0,001 Individual reaction ⬆ ⬇
157 P07737 PROF1_HUMAN Profilin-1 1, 2, 11, 19, 20, 21 Microfilamental network cell-migration related protein [11,43-48] homogeneous upregulation 4,0x T=0,02 homogeneous suppression T=0,05
23-27 P63261 ACTG_HUMAN Actin, cytoplasmic 2 (Gamma-actin) 1, 2, 11, 14, 18, 19, 20, 21 Microfilamental network protein [49-52] homogeneous suppression2x T=0,02 Individual reaction ⬆ ⬇
124 P27797 CRTC_HUMAN Calreticulin precursor CRP55 2, 11, 12, 17, 18, 19, 20, 21 Endoplasmic reticulum calcium-storage protein regulating focal adhesion and cell motility [53-60] homogeneous suppression2x T=0,02 Individual reaction ⬆ ➜ ⬇
155 P30043 BLVRB_HUMAN Flavin reductase, NADHP-dependent reductase 3, 6, 9, 11, 18, 19 Riboflavin biosynthesis pathway [61] individual induction T=0,02 homogeneous induction T=0,002
70-74 P13645 K1C10_HUMAN Keratin, type I cytoskeletal 10 1, 2, 11, 18, 19, 20, 21 Microfilamental network protein [62-67] homogeneous induction T=0,03 homogeneous suppressionT=0,1
136 O00299 CLIC1_HUMAN Chloride intracellular channel protein 1 8, 11, 14, 19, 20, 21 Channel, osmosis, Ca2+-dependent apoptosis-related protein [68-71] 2,5x T=0,04 Individual reaction ⬆ ⬇
156 P08238 HS90B_HUMAN Heat shock protein HSP 90-beta 12, 13, 14, 11, 17, 18, 19, 20, 21 Stress response protein [72-76] homogeneous suppression5x T=0,06 homogeneous induction T=0,02
141 P13489 RINI_HUMAN Placental ribonuclease inhibitor 3, 9, 12, 14, 17, 20, 21 RNA/nucleotide turnover pathway [77-83] homogeneous suppression3x T=0,06 homogeneous suppressionT=0,1
82 P62937 PPIA_HUMAN Peptidyl-prolyl cis-trans isomerase A 4, 11, 12, 14, 17, 19, 20, 21 Cyclophilin A is involved in protein folding, assembly, transportation [84-89] homogeneous suppression3x T=0,06 Individual reaction ⬆ ➜ ⬇
28 not identified protein highly upregulated in several MKs T=0,06 Individual reaction ⬆ ⬇
53 not identified protein highly upregulated in several MKs T=0,06 Individual reaction ⬆ ⬇
142 P08670 VIME_HUMAN Vimentin 1, 2, 11, 14, 18, 19, 20, 21 Microfilamental network cell-migration related protein [60,76,90-97] 2x T=0,09 Individual reaction ⬆ ➜ ⬇
62, 85 93-95 P00915 CAH1_HUMAN Carbonic anhydrase I 5, 11, 18 19, 20, 21 Energy metabolism related protein [98-104] 2x T=0,10 Individual reaction ⬆ ⬇
143 P28838 AMPL_HUMAN Cytosol aminopeptidase 4, 11, 14 19, 20, 21 Regulatory protein-modification enzyme [105-109] individual induction T=0,1 homogeneous suppressionT=0,1
135 P49411 EFTU_HUMAN Elongation factor Tu, mitochondrial precursor 7, 20 Mitochondrial protein synthesis machinery, critical role to maintain the translational fidelity [110,111] homogeneous suppression4x T=0,1 Individual reaction ⬆ ➜ ⬇
45-46 P52566 GDIS_HUMAN Rho GDP-dissociation inhibitor 2 (Rho GDIß) 1, 2, 11, 12, 14, 17, 19, 20, 21 LyDGI plays a role in the onset of apoptosis and cell migration [11,112-116] homogeneous upregulation T=0,1 Individual reaction ⬆ ⬇
148 P63104 1433Z_HUMAN 14-3-3 protein zeta/delta (protein kinase C inhibitor) 11, 12, 14, 17, 18, 19, 20, 21 Cell-cycle checkpoint, stress response protein [117-119] homogeneous suppression2,5x T=0,1 homogeneous suppressionT=0,001
67 P06702 S10A9_HUMAN Protein S100-A9, Calgranulin 2, 11, 14, 18, 19, 20, 21 Ca2+-dependent cell-migration related protein [11,120-127] 2,5x T=0,11 Individual reaction ⬆ ➜ ⬇
110 not identified protein highly upregulated in several MKsT=0,11 Individual reaction ⬆ ⬇
123 P07237 PDIA1_HUMAN Protein disulfide-isomerase precursor, PDI 4, 14, 9, 17, 18, 20, 21 Stress-related protein modification enzyme [60,128-131] 2,5x T=0,12 Individual reaction ⬆ ⬇
104 not identified protein highly upregulated in several MKsT=0,12 Individual reaction ⬆ ⬇
CATEGORY B: non-significantly altered expression profiles in patients versus controls
131 P78371 TCPB_HUMAN T-complex protein 1 subunit beta 4, 20, 21 A member of chaperons family [132,133] individual upregulation ⬆ 2x T=0,15 homogeneous suppressionT=0,05
19-21, 39 P60709 ACTB_HUMAN Actin, cytoplasmic 1 (Beta-actin) 1, 2, 11, 14, 18, 19, 20, 21 Microfilamental network protein [11] slightly increased ⬆ 1,5x T=0,16 Individual reaction ⬆ ➜ ⬇
97 P60174 TPIS_HUMAN Triosephosphate isomerase 5, 7, 19, 20, 21 Energy metabolism related protein [134-137] individual upregulation ⬆ 2x T=0,2 Individual reaction ⬆ ⬇
44 ANXA1-HUMAN Annexin A1 (Calpactin II) 9, 11, 14, 17, 18, 19, 20, 21 Ca2+-dependent phospholipid-binding proteins, potential anti-inflammatory activity [138-141] individual upregulation ⬆ 2x T=0,2 homogeneous induction T=0,1
80 P05109 S10A8_HUMAN Protein S100-A8, Calgranulin 2, 11, 14, 18, 19, 20, 21 Ca2+-dependent cell-migration / tumour related protein [11,120-127] homogeneous ⬆ 2,0x T=0,24 Individual suppression ⬇
37 P47756 CAPZB_HUMAN F-actin-capping protein subunit beta (CapZ beta) 1, 2, 11, 14, 18, 19, 20, 21 Microfilamental network protein [142-145] slightly increased ⬆ T=0,2 Individual reaction ⬆ ⬇
137 P30041 PRDX6_HUMAN Peroxiredoxin-6 9, 10, 11, 14, 17, 18, 19, 20, 21 Multifuctional anti-oxidant, defence, tumour-invasion and metastases related protein [146-150] slightly increased T=0,2 Individual reaction ⬆ ⬇
9-11 P02679 FIBG_HUMAN Fibrinogen gamma chain 11, 17, 19, 20, 21 Microfilamental network cell-migration related protein [151-157] homogeneous ⬆ 1,5x T=0,24 Individual reaction ⬆ ➜ ⬇
130 P10809 CH60_HUMAN 60 kDa heat shock protein 4, 5, 7, 11, 13, 17, 19, 20, 21 Mitochondrial stress response protein,protein-folding [158-166] slightly increased homogeneous level T=0,25 homogeneous suppressionT=0,1
36 Q5U077 Q5U077_HUMAN L-lactate dehydrogenase B 5, 7, 11, 19, 20, 21 Energy metabolism related protein [134,167-172] slightly increased ⬆ 1,5x Individual reaction ⬆ ⬇
122 Q96C61 Q96C61_HUMAN FLNA protein 1, 2, 11, 14, 19, 20, 21 Filamin A - actin binding protein has essential role in intercellular junctions [173-178] homogeneous ⬆ 1,5x Individual reaction ⬆ ⬇
151 P07996 TSP1_HUMAN Thrombospondin-1 precursor 2, 15, 11, 14, 17, 19, 20, 21 The matricellular protein regulating cell adhesion and motility during tissue remodelling, in fibrogenesis & angiogenesis [179-189] Individual induction ⬆ T=0,29 Individual reaction ⬆ ⬇
CATEGORY C: individual group-heterogeneous expression profiles in patients versus homogeneous one in controls
86,87 P00918 CAH2_HUMAN Carbonic anhydrase II 5, 11, 18, 19, 20, 21 Energy metabolism related protein [98-100,103,104,190,191] Individual heterogeneous Individual reaction ⬆ ⬇
103 P02675 FIBB_HUMAN Fibrinogen beta chain precursor 11, 17, 19, 20, 21 Microfilamental network cell-migration related protein [151-157] Individual heterogeneous Individual reaction ⬆ ➜ ⬇
117-120 O75083 WDR1_HUMAN WD repeat-containing protein 1 4, 12, 11, 14, 20 Cell-cycle and proteolytic machinery related protein [189,192] Individual heterogeneous Individual reaction ⬆ ➜ ⬇
126 P28331 NUAM_HUMAN NADH-ubiquinone oxidoreductase 75 kDa 5, 7, 9, 11, 14, 19, 20, 21 Mitochondrial energy metabolism related protein [193-197] highly heterogeneous Individual reaction ⬆ ⬇
127 P08133 ANXA6_HUMAN Annexin A6 (P70) 2, 8, 11, 14, 16, 17, 19, 20, 21 Membrane architecture and signalling protein [127,198-201] Individual induction Individual induction
128 P11142 HSP7C_HUMAN Heat shock cognate 71 kDa protein 4, 5, 11, 13, 14, 17, 18, 19, 20, 21 Stress response protein,chaperone, ATPase [202-206] Individual heterogeneous Individual reaction ⬆ ➜ ⬇
144 Q53XX6 ATPA_HUMAN ATP-synthase, H+ transporting mitochondrial protein 5, 7, 8, 11, 18, 19, 20, 21 Mitochondrial energy metabolism related protein [207-212] Individual heterogeneous Individual reaction ⬆ ⬇
147 P31946 1433B_HUMAN 14-3-3 protein beta/alpha (protein- kinase-C inhibitor) 4, 11, 12, 14, 17, 19, 20, 21 Cell-cycle checkpoint, stress response protein [118,213-216] highly heterogeneous homogeneous suppression T=0,001
152 P14780 MMP9_HUMAN Matrix metalloproteinase-9 11, 14, 15, 18, 19, 20, 21 MMP9 Multifunctional tissue-remodeling protein [217-222] highly heterogeneous Individual reaction ⬆ ⬇
CATEGORY D: similar expression-profiles among patients and controls
1-5 P18206 VINC_HUMAN Vinculin Cytoskeletal assembly associated protein similar Individual reaction ⬆ ⬇
6-8, 17, 34, 38, 63, 105, 109, 111 not identified protein spots similar Individual reaction ⬆ ⬇
12,13, 32, 33, 43, 47, 48, 98 P60709 ACTB_HUMAN Actin, cytoplasmic 1 (Beta-actin) Microfilamental network protein similar Individual reaction ⬆ ⬇
14-15 P68363 TBA1B_HUMAN Tubulin alpha- chain Microtubule network protein similar Individual reaction ⬆ ⬇
16 P06576 ATPB_HUMAN ATP synthase subunit beta, mitochondrial precursor Mitochondrial energy metabolism related protein similar Individual reaction ⬆ ⬇
18 P07437 TBB2_HUMAN Tubulin beta-2 chain Microfilamental network protein similar Individual reaction ⬆ ➜ ⬇
29-31, 51, 52, 154–61, 64, 79, 81, 83, 84, 89-92 P13645 K1C10_HUMAN Keratin, type I cytoskeletal 10 Microfilamental network protein similar Individual reaction ⬆ ⬇
40, 41 P63261 ACTG_HUMAN Actin, cytoplasmic 2 (Gamma-actin) Microfilamental network protein similar Individual reaction ⬆ ⬇
42 Q6FHP5_HUMAN PHB protein Prohibitin - negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. similar homogeneous suppression ⬇
49-50 P67936 TPM4_HUMAN Tropomyosin alpha-4 chain Microfilamental network protein similar Individual reaction ⬆ ⬇
88 P02768 ALBU_HUMAN Serum albumin Extracellular transport/carrier protein similar Individual reaction ⬆ ⬇
96 P18669 PGAM1_HUMAN Phosphoglycerate mutase 1 Energy metabolism related protein similar homogeneous suppression T=0,1
99 P00558 PGK1_HUMAN Phosphoglycerate kinase 1 Energy metabolism related protein similar Individual reaction ⬆ ➜ ⬇
100 P68871 HBB_HUMAN Hemoglobin subunit beta Oxygen carrier similar Individual reaction ⬆ ⬇
101, 102, 106 P06733 ENOA_HUMAN Alpha-enolase multifunctional glycolytic enzyme similar Individual reaction ⬆ ➜ ⬇
107 P14618 KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 Energy metabolism related protein similar Individual reaction ⬆ ➜ ⬇
125 P14625 ENPL_HUMAN Endoplasmin precursor (94-kDa glucose-regulated protein) Signal transduction pathways associated with endoplasmic reticulum stress similar homogeneous suppressionT=0,1
129 P13796 PLSL_HUMAN Plastin-2 Microfilamental network protein similar homogeneous suppression T=0,02
132 Q53QM2 Q53QM2_HUMAN Hypothetical protein ACTR3 Currently uncharacterized protein similar homogeneous suppression T=0,1
133 Q6IAT1 Q6IAT1_HUMAN GDI2 protein (GDP dissociation inhibitor 2) Regulatory protein in the functional cycle and recycling of Rab GTPases similar Individual suppression ⬇
134 UQCR1_HUMAN Reductase complex core protein I Ubiquinol-cytochrome C- reductase, mitochondrial processing peptidase Beta-family similar Individual reaction ⬆ ⬇
138 P09211 GSTP1_HUMAN Glutathione S-transferase P (GST class-pi) Stress response and anti-oxidant defence protein similar homogeneous induction T=0,07
139 P07741 APT_HUMAN Adenine phosphoribosyl-transferase Nucleotide metabolism similar Individual reaction ⬆ ➜ ⬇
140 P11021 GRP78_HUMAN 78 kDa glucose-regulated protein precursor (GRP 78) Energy metabolism related protein similar Individual reaction ⬆ ➜ ⬇
145 P13716 HEM2_HUMAN Delta-aminolevulinic acid dehydratase anti-oxidant defence and detoxification pathways similar homogeneous suppression T=0,07
146 Q5JQI4 HSP71_HUMAN Heat shock 70 kDa protein 1A Stress response protein similar Individual reaction ⬆ ⬇
149 Q06323 PSME1_HUMAN Proteasome activator complex subunit 1 The activator binds to proteasome 20S & enhances peptidase activity, e.g. under stress conditions similar Individual reaction ⬆ ⬇
150 P00491 PNPH_HUMAN Purine nucleoside phosphorylase Nucleotide- and nucleoside turnover, detoxification pathway similar Individual suppression ⬇
153 P12429 ANXA3_HUMAN Annexin A3 Membrane architecture and signalling protein similar Individual reaction ⬆ ➜ ⬇
154 VDAC1_HUMAN Voltage-dependent anion-selective channel protein 1 Membrane protein, regulation of cell growth / death via redox-control similar Individual induction ⬆
158 Q9Y3F5_HUMAN A6-related hypothetical protein Twinfilin-2, Protein tyrosine kinase 9-like, actin-binding protein involved in motile and morphological processes similar Homogeneous suppression T=0,1

Annotation to Table1: 158 spots have been distinguished by protein mapping as stably expressed (i.e. by all members of the group) in circulating leucocytes of the group with breast cancer patients. Altogether 74 proteins have been identified within 158 spots. The protein mapping image is demonstrated in Figure 5. The spot number in the map (Spot number) and corresponding accession number (Access number) and name (Accession name) received from the SwissProt database is provided in the table together with the name of the identified protein (Protein name) in accordance with the current protein nomenclature. The column “Classification” provides information about the function(s) currently known for each protein. The corresponding number of the functional group(s) is/are provided in the column “Functional group number”; the designation of the functional group with the corresponding number can be found in the separate Table 2. The regulation manner (up / down regulation) and the severity of the expression profile alterations under the cancer condition have been qualified and quantified versus the values in the control group; the resulting information is provided in the column “Profile alterations versus controls”. In accordance to the expression profile alterations, every mapped protein has been assigned to one of the altogether four CATEGORIES built-up as follows: A = 22 proteins with the statistically significant alterations in the expression profiles under the cancer condition compared to the control group (T≤0,1); B = 12 with the statistically non-significant alterations in the expression profiles under the cancer condition compared to the control group; C = 9 proteins with the expression profiles altered individually with highly heterogeneous expression profiles within the patient group versus stable expression levels within the control group; D = 31 proteins with similar expression-profiles within both patient and control groups of comparison. Further, under the cancer condition, the expression alterations as the reaction towards the applied radiotherapy has been qualified (up / down regulation) and quantified as it is summarised for each protein in the column “Alterations under radiotherapy”. The resulting statistics is provided here: 14 proteins homogeneously (group-significantly) suppressed (), 4 proteins homogeneously (group-significantly) induced (⬆ ), 4 proteins individually (group-non-significantly) suppressed (), 2 proteins individually (group-non-significantly) induced (⬆ ), 33 individually up- or down-regulated proteins (⬆ ⬇ ), and 17 proteins with individual up-/or down-/or unchanged regulation (⬆ ➜ ⬇ ) have been profiled under radiotherapy.

Golubnitschaja et al.

Golubnitschaja et al. EPMA Journal 2013 4:6   doi:10.1186/1878-5085-4-6

Open Data